منابع مشابه
A new preparation of crystalline anterior pituitary growth hormone.
The isolation and identification of the anterior pituitary growth hormone as a pure protein were first achieved by Li, Evans, and Simpson (1, 2) in 194445. Their method is a beautiful example of the classical procedures of protein fractionation by the controlled use of salt and of hydrogen ion concentrations. It owes its distinction to the proof that the growth-promoting activity of anterior pi...
متن کاملStudies on Pituitary Lactogenic Hormone
Previous investigations have shown that the ovine prolactin is composed of a single chain at the N terminus, with threonine as the terminal residue (1). Since the hormone protein has 3 cystine residues (2, 3), it might be anticipated that one or more of these three -S-Sbridges may form a branched chain in the C terminus or along a portion of the polypeptide chain. If this were the case, oxidati...
متن کاملStudies on Pituitary Lactogenic Hormone
The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic...
متن کاملPreparation of pituitary thyrotropic hormone.
The existence of a thyroid-stimulating hormone in anterior pituitary gland tissue has been known for 30 years (1, 2). During this time, considerable work has been done on the physiological and the pharmacological effects of the thyrotropic hormone administ’ered in the form of pituitary extracts which had been subjected t,o a varying degree of manipulation. Relatively little progress on the puri...
متن کاملPreparation of Pituitary Thyrotropic Hormone*
The existence of a thyroid-stimulating hormone in anterior pituitary gland tissue has been known for 30 years (1, 2). During this time, considerable work has been done on the physiological and the pharmacological effects of the thyrotropic hormone administ’ered in the form of pituitary extracts which had been subjected t,o a varying degree of manipulation. Relatively little progress on the puri...
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ژورنال
عنوان ژورنال: Endocrinology
سال: 1995
ISSN: 0013-7227,1945-7170
DOI: 10.1210/endo.136.4.7895643